Novel enzyme nanoparticles having methane oxidation activity
AssigneeKorea University Research and Business Foundation
InventorsJeewon LEE, Hyun Jin KIM
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Abstract
The present invention relates to novel enzyme nanoparticles capable of converting methane into methanol, in which key active sites of methane-oxidizing bacteria are fused with each other and expressed on a protein that can be self-assembled in cells to form nanoparticles, and specifically to enzyme nanoparticles including a protein having methane monooxygenase (MMO) activity and active sites of the methane oxidase, a method for production thereof, a recombinant microorganism into which a nucleic acid encoding the protein and the active site of the methane oxidase is introduced, and immobilized enzyme nanoparticles including the enzyme nanoparticles loaded on a carrier.
Claims21
An enzyme nanoparticle having methane monooxygenase (MMO) (“methane oxidase”) activity, wherein a first active site and a second active site of the methane oxidase are fused with each other and expressed on a surface of a protein adapted to be self-assembled in cells to form nanoparticles.
(canceled)
A recombinant microorganism having enzyme nanoparticle production ability, into which:
- i) a nucleic acid encoding a protein adapted to be self-assembled in cells to form nanoparticles;
- ii) a nucleic acid encoding a first active site of a methane oxidase;
- and
- iii) a nucleic acid encoding a second active site of the methane oxidase are introduced, wherein the first active site and the second active site are fused with each other and expressed on a surface of protein nanoparticles to exhibit methane monooxygenase (MMO) activity.
(canceled)
Dependencies are inferred from the claim text.