Control of insect pests using rna molecules
AssigneeDevgen NV
InventorsLien DE SCHRIJVER, Kaat CAPPELLE, Lies DEGRAVE, Myriam BEGHYN, Yann NAUDET
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Abstract
Disclosed are double stranded RNA molecules that are toxic to flea beetles, particularly the flea beetle species Psylliodes chrysocephala, Phyllotreta nemorum, Phyllotreta striolata, Phyllotreta armoraciae, Phyllotreta atra and Phyllotreta cruciferae. In particular, interfering RNA molecules capable of interfering with pest target genes and that are toxic to the target pest are provided. Further, methods of making and using the interfering RNA, for example in transgenic plants or as the active ingredient in a composition, to confer protection from insect damage are disclosed.
Claims17
An interfering ribonucleic acid (RNA) molecule wherein the RNA comprises at least one dsRNA wherein the dsRNA is a region of double-stranded RNA comprising annealed complementary strands, one strand of which comprises a sequence of at least 19 contiguous nucleotides which is at least partially complementary to a target nucleotide sequence within a flea beetle target gene, and (i) is at least 85% identical to at least a 19 contiguous nucleotide fragment of SEQ ID NO:
- 409-612, or the complement thereof, (ii) comprises at least a 19 contiguous nucleotide fragment of SEQ ID NO:
- 409-612, or the complement thereof, (iii) comprises at least a 19 contiguous nucleotide fragment of a nucleotide sequence encoding an amino acid sequence encoded by SEQ ID NO:
- 409-612, or the complement thereof, or (iv) can hybridize under stringent conditions to a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
- 409-612, or the complements thereof, wherein the interfering RNA molecule has insecticidal activity on an insect pest, wherein the insect pest is a flea beetle of the Alticini tribe.
A transgenic plant, or part thereof, comprising the interfering RNA molecule, the nucleic acid molecule, the nucleic acid construct, and/or the composition of any of the respective preceding claims, wherein the transgenic plant has enhanced resistance to a flea beetle as compared to a control plant.
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Dependencies are inferred from the claim text.