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US 2022/0169992 A1Published application
Optimized parvovirus h-1 production
AssigneeDeutsches Krebsforschungszentrum Stiftung des Offentlichen Rechts
InventorsBarbara LEUCHS, Veronika FREHTMAN, Marcus MÜLLER, Jean ROMMELAERE, Michael DAHM, Ottheinz KREBS
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Abstract
The present invention provides a robust single clone Master Cell Bank (MCB) for an optimized production of H-1 parvovirus (H-1 PV) which is suitable to increase infectious parvovirus production compared to standard producer NB-324K mixed cells.
Claims9
Process for preparing a single clone master cell bank (MCB) for parvovirus H-1PV production, comprising the following steps:
- (a) growing a NB-324K mixed clone in a cell culture medium,
- (b) seeding ideally 1 cell per well in a 96 well plate and growing it for 20-28 days,
- (c) choosing 2 to 5 cell clones obtained in step (b), in which the cells showed a single colony per well,
- (d) seeding at least two first round single clones in a 96 well as single cell/well for 20-23 days, and testing for growth and production,
- (e) propagating and passaging the selected single cell clone with the best growth and production properties until a passage higher than passage 15,
- (f) harvesting and storing the obtained cells.
3 more elements
Dependencies are inferred from the claim text.