Composition for tissue regeneration, method of production and uses thereof

A method for obtaining a composition for tissue regeneration, comprising the following steps:

• providing M2-macrophages, wherein this step of providing M2-macrophages comprises purifying monocytes from a sample and differentiating the purified monocytes into M2-macrophages;
• co-culturing the M2-macrophages with tissue-specific cells in serum free medium;
• and
• collecting the supernatant of the co-culture,
• wherein the ratio of M2-macrophages to tissue-specific cells in the co-culture is 1:1 to 40:1.

A composition obtained by a method for obtaining a composition for tissue regeneration consisting of the following steps:

• providing M2-macrophages, wherein this step of providing M2-macrophages comprises purifying monocytes from a sample and differentiating the purified monocytes into M2-macrophages;
• co-culturing the M2-macrophages with tissue-specific cells in serum free medium;
• and
• collecting the supernatant of the co-culture,
• wherein the ratio of M2-macrophages to tissue-specific cells in the co-culture is 1:1 to 40:1, wherein the tissue-specific cells are selected from the group comprising myocytes, skeletal muscle cells, muscle satellite cells, tenocytes, osteocytes, Schwann cells, dermal fibroblasts, chondrocytes, synoviocytes, lymphocytes, hair follicle cells, myocardia cells, cells from respiratory epithelium, lymphatic endothelial cells or mesenchymal stem cells or combinations thereof and wherein the M2-macrophages are co-cultured with the tissue-specific cells for a period of 3 to 28 days.

A method for modifying the growth factor and/or cytokine composition of the culture medium in a M2-macrophage culture, comprising:

• providing M2-macrophages, wherein this step of providing M2-macrophages comprises purifying monocytes from a sample and differentiating the purified monocytes into M2-macrophages;
• and
• co-culturing the M2-macrophages with tissue-specific cells in serum free medium,
• wherein the growth factor and/or cytokine composition of the culture medium is modified as compared to the growth factor and/or cytokine composition of a culture medium in a M2-macrophage culture without the tissue-specific cells in serum free medium, wherein the ratio of M2-macrophages to tissue-specific cells in the co-culture is 1:1 to 40:1.