Polypeptides having beta-glucanase activity, polynucleotides encoding same and uses thereof in cleaning and detergent compositions

A polypeptide having beta-glucanase activity, wherein the polypeptide is a gram-positive bacteria of order Bacillales and comprises a motif selected from the group consisting of NXAXGG (SEQ ID NO:

• 30), GEXDXME (SEQ ID NO:
• 28), GXGNXEXXXY (SEQ ID NO:
• 29), YTS[G/A][K/R] (SEQ ID NO:
• 31) and combinations thereof.

A polypeptide having beta-glucanase activity, selected from the group consisting of:

• a) a polypeptide having at least 97.5%, at least 98%, at least 98.5%, at least 99%, at least 99.5%, or even 100% sequence identity to the polypeptide of SEQ ID NO:
• 12, a polypeptide having at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or even 100% sequence identity to the polypeptide of SEQ ID NO:
• 3, a polypeptide having at least 98.5%, at least 99%, at least 99.5%, or even 100% sequence identity to the polypeptide of SEQ ID NO:
• 6, a polypeptide having at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or even 100% sequence identity to the polypeptide of SEQ ID NO:
• 9, a polypeptide having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or even 100% sequence identity to the polypeptide of SEQ ID NO:
• 15, a polypeptide having at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or even 100% sequence identity to the polypeptide of SEQ ID NO:
• 18;
• b) a polypeptide having at least 97.5%, at least 98%, at least 98.5%, at least 99%, at least 99.5%, or even 100% sequence identity to the mature polypeptide of SEQ ID NO:
• 11, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or even 100% sequence identity to the mature polypeptide of SEQ ID NO:
• 2, at least 98.5%, at least 99%, at least 99.5%, or even 100% sequence identity to the mature polypeptide of SEQ ID NO:
• 5, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or even 100% sequence identity to the mature polypeptide of SEQ ID NO:
• 8, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or even 100% sequence identity to the mature polypeptide of SEQ ID NO:
• 14, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or even 100% sequence identity to the mature polypeptide of SEQ ID NO:
• 17;
• c) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions with (i) the mature polypeptide coding sequence of the sequence selected from the group consisting of:
• SEQ ID NO:
• 10, SEQ ID NO:
• 1, SEQ ID NO:
• 4, SEQ ID NO:
• 7, SEQ ID NO:
• 13, SEQ ID NO:
• 16, or (ii) the full-length complement of (i);
• d) a polypeptide encoded by a polynucleotide having at least 97.5%, at least 98%, at least 98.5%, at least 99%, at least 99.5%, or even 100% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 10, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or even 100% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 1, at least 98.5%, at least 99%, at least 99.5%, or even 100% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 4, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or even 100% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 7, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or even 100% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 13, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or even 100% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 16;
• e) a variant of a polypeptide of the sequence selected from the group consisting of SEQ ID NO:
• 12, SEQ ID NO:
• 3, SEQ ID NO:
• 6, SEQ ID NO:
• 9, SEQ ID NO:
• 15, and SEQ ID NO:
• 18;
• f) a variant of the mature polypeptide of the sequence selected from the group consisting of:
• SEQ ID NO:
• 11, SEQ ID NO:
• 2, SEQ ID NO:
• 5, SEQ ID NO:
• 8, SEQ ID NO:
• 14, SEQ ID NO:
• 17, wherein said variant comprising a substitution, deletion, and/or insertion at one or more positions;
• g) and a fragment of the polypeptide of (a), (b), (c), (d), (e), or (f) that has beta-glucanase activity.