Direct detection method for tick-borne infections and a cell technology medium for the method

A method for detecting tick-borne infections in human/animal body fluid of a subject, the human/animal body fluid comprising a blood sample, the method comprising:

• adding the blood sample directly into a specified amount of cell technology medium establishing a partially hostile (semi-hostile) environment due to its low-level antibiotic or preservative content aiming at bacteriostatic effect and lowered sugar content, mixing the blood sample and the cell technology medium, and, if required, incubating and storing the blood sample and the cell technology medium, wherein, cells and pathogens of the subject are in the semi-hostile environment, separating corpuscles and pathogens by centrifuging with a low-speed of 2-4000 g, thus obtaining plasma, blood cells and a separating layer called “buffy coat,” removing the plasma, mixing a small amount of blood cells from said “buffy coat” layer and blood cells located a few cell layers beneath it with the plasma, concentrating the sample, and testing to detect pathogens in the plasma and in blood cells.