Methods of improving production of morphinan alkaloids and derivatives
OwnerChristina SMOLKE, Catherine THODEY +3inventors
InventorsChristina SMOLKE, Catherine THODEY, Kristy HAWKINS, Xuezhi LI, Amy KOZINA
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Abstract
Methods and systems are provided for producing codeinone within an engineered non-plant cell. The method comprises, within the engineered non-plant cell, producing a thebaine product. The method also comprises, within the engineered non-plant cell, contacting the thebaine product with an enzyme having thebaine 6-O-demethylase activity, thereby producing a neopinone product. Additionally, the method comprises, within the engineered non-plant cell, contacting the neopinone product with a neopinone isomerase, thereby producing a codeinone product.
Claims18
A method of producing codeinone within an engineered non-plant cell, the method comprising:
- within the engineered non-plant cell, producing a thebaine product;
- within the engineered non-plant cell, contacting the thebaine product with an enzyme having thebaine 6-O-demethylase activity, thereby producing a neopinone product;
- and
- within the engineered non-plant cell, contacting the neopinone product with a neopinone isomerase, thereby producing a codeinone product.
A method of isomerizing neopinone within an engineered non-plant cell, the method comprising:
- contacting a neopinone product with a neopinone isomerase,
- wherein contacting the neopinone product with the neopinone isomerase isomerizes the neopinone product to a codeinone product, and
- wherein the neopinone isomerase is produced by culturing an engineered non-plant cell having at least one coding sequence, encoded within a chromosome within the engineered non-plant cell, for encoding the neopinone isomerase.
Dependencies are inferred from the claim text.