Engineered deoxyribose-phosphate aldolases
AssigneeCodexis, Inc.
InventorsDa Duan, Oscar Alvizo, Jovana Nazor, Harvinder Chagger Maniar, James Nicholas Riggins, Jonathan Vroom, Santhosh Sivaramakrishnan, Hao Yang, Anna Fryszkowska, Mark A. Huffman, Joshua N. Kolev, Iman Farasat, Agustina Rodriguez-Granillo, Deeptak Verma
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Abstract
The present invention provides engineered deoxyribose-phosphate aldolase polypeptides useful under industrial process conditions for the production of pharmaceutical and fine chemical compounds.
Claims17
An engineered polynucleotide encoding an engineered deoxyribose-phosphate aldolase, wherein said engineered deoxyribose-phosphate aldolase comprises a polypeptide sequence having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to SEQ ID NOs:
- 2, 6, and/or 466, or a functional fragment thereof, wherein said engineered deoxyribose-phosphate aldolase comprises at least one substitution or substitution set in said polypeptide sequence, and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NOs:
- 2, 6, and/or 466.
An engineered polynucleotide sequence encoding at least one engineered deoxyribose-phosphate aldolase, wherein said polynucleotide sequence comprises at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to SEQ ID NOs:
- 1, 5, and/or 465, wherein the polynucleotide sequence of said engineered deoxyribose-phosphate aldolase comprises at least one substitution at one or more positions.
An engineered polynucleotide sequence encoding at least one engineered deoxyribose-phosphate aldolase comprising at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to SEQ ID NOs:
- 1, 5, and/or 465, or a functional fragment thereof.
Dependencies are inferred from the claim text.