Bacterium And Obtaining Method And Application Thereof

A method for coproducing 1,3-propanediol and D-lactic acid by fermenting an artificial bacterium, wherein the artificial bacterium has a property of coproducing 1,3-propanediol and D-lactic acid and is obtained through modification of a natural bacterium;

• wherein the natural bacterium is Klebsiella oxytoca PDL-0, the Klebsiella oxytoca PDL-0 was collected in China Center for Type Culture Collection on Apr. 8, 2016, and the collection registration number is CCTCC M 2016184;
• wherein the modification is defecting one or more genes of budA, budB, adhE, ackA-pta, poxB and frdA of the natural bacterium.

A bacterium, wherein the bacterium has a property of coproducing 1,3-propanediol and D-lactic acid, wherein the bacterium is Klebsiella oxytoca PDL-0, the Klebsiella oxytoca PDL-0 was collected in China Center for Type Culture Collection on Apr. 8, 2016, and the collection registration number is CCTCC M 2016184.

An artificial bacterium, wherein the artificial bacterium has a property of coproducing 1,3-propanediol and D-lactic acid and is obtained through modification of a natural bacterium, wherein the modification comprises introducing an exogenous 1,3-PD synthesis pathway and/or D-LAC synthesis pathway into the natural bacterium;

• wherein the step of introducing the exogenous 1,3-PD synthesis pathway comprises transforming a glycerol dehydratase coding gene dhaB and a 1,3-PD oxidoreductase coding gene dhaT into the natural bacterium;
• wherein the step of introducing the exogenous D-LAC synthesis pathway comprises performing codon optimization applicable to expression of the natural bacterium to a gene dldhBc of D-lactate dehydrogenase and replacing the D-lactate dehydrogenase into a position of D-lactate dehydrogenase in a genome of natural bacterium by using a shuttle plasmid, so as to realize constitutive expression of dldhBc on the genome of the natural bacterium.