Production and uses of artifical histone h1 for analyzing, diagnosing, treating, and/or preventing senescence

A method for producing an artificial protein sequence for histone H1 variants to induce resistance and/or protection against senescence, and/or age-related health conditions wherein the method comprises the steps of:

• a. selecting a wild-type histone H1.0 or H1x protein sequence, or the wild-type sequence of a respective protein ortholog in the species of interest;
• b. within the sequence selected in step a, recognizing the subsequences determined by regions or individual sites in the globular domain of the protein that conform the DNA-binding site of the histone H1.0 or H1x proteins, particularly the amino acid residues directly or indirectly interacting with the DNA;
• c. applying a set of at least one amino acid substitutions, insertions, and/or deletions to one or more of the amino acid subsequences corresponding to the regions or sites recognized in step b, where the modifications do not alter the structure of the α-helical motifs and where the respective net electric charge (z) associated to each resulting modified amino acid subsequence is greater than before the modifications;
• and
• d. obtaining the artificial protein sequence by applying the set of at least one amino acid substitutions, insertions, and/or deletions determined by step c into the wild-type histone H1.0, histone H1x, or respective orthologous protein sequence selected in step a, thereby producing the complete artificial protein sequence.