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US 2022/0162251 A1Published application
Nucleotide analogues
AssigneeIsoPlexis Corporation
InventorsMong Sano MARMA, Jerzy Olejnik, Ilia Korboukh
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Abstract
The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.
Claims10
-47. (canceled)
A composition comprising:
- i) at least one deoxynucleoside triphosphate according to the following structure:
- wherein B is a nucleobase;
- and
- ii) at least one labeled deoxynucleoside triphosphate according to the following structure:
- wherein D is selected from the group consisting of an azide, disulfide alkyl, disulfide substituted alkyl groups, disulfide allyl, and disulfide substituted allyl groups;
- B is a nucleobase;
- Linker comprises a cleavable oxymethylenedisulfide-containing site core, wherein said cleavable site core is selected from the group consisting of:
- wherein R1 and R2 are independently selected alkyl groups;
- and Label is a label.
6 more elements
Dependencies are inferred from the claim text.