Droplet-based surface modification and washing
AssigneesAdvanced Liquid Logic, Inc., Duke University
InventorsVamsee K. Pamula, Vijay Srinivasan, Michael G. Pollack, Richard B. Fair
Life of this patent
Estimating the term…
Abstract
The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.
Claims21
-23. (canceled)
A method comprising:
- immobilizing on a surface an antibody with specificity for a target analyte;
- washing the immobilized antibody to remove excess antibody;
- using droplet operations to expose the immobilized antibody to a sample droplet;
- washing the immobilized antibody to remove unbound components of the sample droplet;
- exposing the immobilized antibody to a droplet including a reporter antibody;
- washing away excess reporter antibody;
- and
- measuring a measurable parameter or signal facilitated by the reporter antibody.
An apparatus comprising:
- two parallel substrates separated by a gap, where one or both of the substrates comprise electrodes, where the electrodes are capable of conducting one or more droplet operations, where the electrodes are electronically coupled to and controlled by a controller.
An apparatus comprising:
- a plurality of reservoirs, where each reservoir comprises one or more reagents;
- a droplet microactuator, where the droplet microactuator comprises two parallel substrates separated by a gap, where one or both of the substrates comprise electrodes, where the electrodes are capable of conducting one or more droplet operations, where the electrodes are electronically coupled to and controlled by a controller;
- and
- a fluidic path between the plurality of reservoirs and the droplet microactuator.
Dependencies are inferred from the claim text.