Artificial virus presenting cells
AssigneeDiagnologix, LLC
InventorsYu-Tsueng LIU, Guixin SHI, Chuntang FU
Life of this patent
Estimating the term…
Abstract
A method for ex vivo transduction of biomolecules from viruses, viral vectors or virus-like particles into target cells and microbubbles for use in this method. A quantity of viruses, viral vectors or virus-like particles and target cells are bound to flexible lipid shell microbubbles, bringing these into close proximity to each other that allows viral transduction, transferring biomolecules from the viruses, viral vectors or virus-like particles into the target cells while the viruses, viral vectors or virus-like particles and the target cells are bound to the microbubbles.
Claims21
A method for ex vivo transduction of biomolecules from viruses, viral vectors or virus-like particles into target cells, comprising:
- preparing a mixture by mixing a quantity of viruses, viral vectors or virus-like particles and flexible lipid shell microbubbles, said flexible lipid shell microbubbles being conjugated with one or more ligands binding to the viruses, viral vectors or virus-like particles and to the target cells;
- incubating the mixture over a time span allowing the viruses, viral vectors or virus-like particles to bind to microbubbles;
- incubating the microbubbles with the viruses, viral vectors or virus-like particles and the target cells to allow transduction to take place, transferring the biomolecules from viruses, viral vectors or virus-like particles into the target cells while the viruses, viral vectors or virus-like particles and the target cells are bound to the microbubbles.
Flexible lipid shell microbubbles adapted to facilitate viral transduction between viruses, viral vectors or virus-like particles and target cells, transferring biomolecules from the viruses, viral vectors or virus-like particles into the target cells while the viruses, viral vectors or virus-like particles and the target cells are bound to the microbubbles, wherein the flexible lipid shell microbubbles are conjugated with bi-specific ligands that are capable of binding to both the viruses, the viral vectors or virus-like particles and the target cells or are conjugated with at least a first and a second ligand differing from each other with the first ligand binding to the viruses or the viral vectors but not to the target cells and the second ligand binding to the target cells but not to the viruses, the viral vectors or virus-like particles.
Dependencies are inferred from the claim text.