Methods and kits for detecting tau
AssigneesMeso Scale Technologies, LLC., The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., The United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsChristopher CAMPBELL, Kimbra KENNEY, Jessica GILL
Life of this patent
Estimating the term…
Abstract
The invention relates to methods and kits for assessing brain injury, e.g., traumatic brain injury resulting from blast exposure. The invention provides methods of quantifying the amount of phosphorylated tau or total tau in a biological sample. The invention further provides a method of determining the number of blast exposures experienced by a subject. Also provided herein are kits for detecting phosphorylated tau or total tau in a biological sample.
Claims37
A method of quantifying the amount of phosphorylated tau (p-tau) in a biological sample, wherein the method comprises:
- a) contacting the biological sample with:
- (i) a first capture reagent that binds non-phosphorylated tau;
- (ii) a second capture reagent that binds non-phosphorylated tau, wherein the first and second capture reagents are on a surface, and the surface further comprises an anchoring reagent;
- (iii) a first detection reagent that binds non-phosphorylated tau, wherein the first detection reagent comprises a first nucleic acid probe;
- and (iv) a second detection reagent that binds p-tau, wherein the second detection reagent comprises a second nucleic acid probe,
- thereby forming a complex on the surface comprising the first and second capture reagents, p-tau, and the first and second detection reagents;
- b) using an extension process that requires the first and second nucleic acid probes to be in proximity, extending the second nucleic acid probe to form an extended sequence comprising an anchoring region that binds to the anchoring reagent;
- c) binding the extended sequence to the anchoring reagent;
- and
- d) measuring the amount of extended sequence bound to the surface, thereby quantifying the amount of p-tau;
- or wherein the method comprises:
- a) contacting the biological sample with:
- (i) a first capture reagent that binds non-phosphorylated tau;
- (ii) a second capture reagent that binds non-phosphorylated tau, wherein the first and second capture reagents are on a surface, and the surface further comprises an anchoring reagent (iii) a detection reagent that binds p-tau, wherein the detection reagent comprises a nucleic acid probe,
- thereby forming a complex on the surface comprising the first and second capture reagents, p-tau, and the detection reagent;
- b) extending the nucleic acid probe to form an extended sequence comprising an anchoring region that binds to the anchoring reagent;
- c) binding the extended sequence to the anchoring reagent;
- and
- d) measuring the amount of extended sequence bound to the surface, thereby quantifying the amount of p-tau,
- or wherein the method comprises:
- a) contacting the biological sample with:
- (i) a first capture reagent that binds non-phosphorylated tau;
- (ii) a second capture reagent that binds non-phosphorylated tau, wherein the first and second capture reagents are on a surface;
- (iii) a detection reagent that binds p-tau, wherein the detection reagent comprises a detectable label,
- thereby forming a complex on the surface comprising the first and second capture reagents, p-tau, and the detection reagent;
- and
- b) measuring the amount of detectable label on the surface, thereby quantifying the amount of p-tau.
(canceled)
(canceled)
(canceled)
8-10. (canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
A method of determining the number of blast exposures experienced by a subject, comprising
- a) quantifying the amount of total tau (t-tau) in a biological sample of the subject;
- and
- b) determining the number of blast exposures experienced by the subject based on the amount of t-tau.
(canceled)
(canceled)
(canceled)
35-38. (canceled)
A kit for detecting phosphorylated tau (p-tau) in a biological sample, wherein the kit comprises, in one or more vials, containers, or compartments:
- a) a surface;
- b) a first capture reagent that binds non-phosphorylated tau;
- c) a second capture reagent that binds non-phosphorylated tau;
- d) a first detection reagent that binds non-phosphorylated tau;
- and
- e) a second detection reagent that binds p-tau;
- or wherein the kit comprises, in one or more vials, containers, or compartments:
- a) a surface;
- b) a first capture reagent that binds non-phosphorylated tau;
- c) a second capture reagent that binds non-phosphorylated tau;
- and
- d) a detection reagent that binds p-tau.
40-64. (canceled)
A kit for detecting total tau (t-tau) in a biological sample, wherein the kit comprises, in one or more vials, containers, or compartments:
- a) a first capture reagent that binds tau and that is immobilized to or capable of being immobilized to the surface;
- b) a second capture reagent that binds tau and that is immobilized to or capable of being immobilized to the surface;
- c) a first detection reagent that binds tau and that comprises a first nucleic acid probe or is capable of being conjugated to a first nucleic acid probe;
- and
- d) a second detection reagent that binds tau and that comprises a second nucleic acid probe or is capable of being conjugated to a second nucleic acid probe;
- or wherein the kit comprises, in one or more vials, containers, or compartments:
- a) a first capture reagent that binds tau and that is immobilized to or capable of being immobilized to the surface;
- b) a second capture reagent that binds tau and that is immobilized to or capable of being immobilized to the surface;
- and
- c) a detection reagent that binds tau and that comprises a nucleic acid probe or a detectable label, or that is capable of being conjugated to a nucleic acid probe or a detectable label.
66-68. (canceled)
Dependencies are inferred from the claim text.