Methods and systems for processing or analyzing oligonucleotide encoded molecules

A method of determining a target-activity of at least one resolved oligonucleotide encoded molecule comprising:

• providing a separation medium, wherein the separation medium contains at least one target molecule;
• introducing a sample containing a mixture of at least two different oligonucleotide encoded molecules to the separation medium, wherein the at least two different oligonucleotide encoded molecules include an encoding portion operatively linked to at least one encoded portion;
• forming at least two different resolved oligonucleotide encoded molecules by separating the at least two different oligonucleotide encoded molecules into at least two separate locations in the separation medium;
• harvesting the at least one resolved oligonucleotide encoded molecule from the at least two different resolved oligonucleotide encoded molecules by segmenting at least one location of the at least two separate locations from the separation medium to form at least one resolved segment;
• processing the at least one resolved oligonucleotide encoded molecule to allow for performing polymerase chain reaction (PCR);
• amplifying the at least one encoded portion of the at least one resolved oligonucleotide encoded molecule by performing PCR on the encoding portion of the at least one resolved oligonucleotide encoded molecule;
• and
• determining a target-activity of the at least one resolved oligonucleotide encoded molecule by processing the at least one location and an identity of the at least one encoded portion of the at least one resolved oligonucleotide encoded molecule.