Materials and methods for reducing nucleic acid degradation in bacteria
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsValerie Anne De Crecy, Geoffrey Jean Hutinet
Life of this patent
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Abstract
The present disclosure is directed to materials and methods for reducing heterologous DNA damage in bacteria (i.e., induce resistance to host restriction enzymes) by modifying the heterologous DNA to include one or more deazapurine bases.
Claims15
A bacterial cell comprising a heterologous nucleic acid sequence comprising one or more deazapurine bases.
A method of protecting a heterologous nucleic acid sequence from cleavage by restriction enzymes in a host bacterium, the method comprising:
- modifying the heterologous nucleic acid sequence to incorporate one or more deazaguanine bases;
- and
- introducing the modified heterologous nucleic acid sequence into the host bacterium, thereby protecting the heterologous nucleic acid sequence from cleavage by restriction enzymes in the host bacterium.
A method of producing a bacteriophage composition, the method comprising (a) modifying a nucleic acid of bacteriophage origin to incorporate one or more deazaguanine bases;
- (b) introducing the modified nucleic acid into a host bacteria cell;
- (c) incubating the host bacteria cell until phage-mediated bacterial lysis occurs;
- and (d) isolating bacteriophage lysate.
Dependencies are inferred from the claim text.