Targeting Vector, Nucleic Acid Composition, and Method for Constructing Liver-injured Mouse Model

A targeting vector for constructing a liver-injured mouse model, wherein the targeting vector contains a target sequence, and a 5′ end homologous arm sequence and a 3′ end homologous arm sequence for mediating insertion of the target sequence into a target site in a mouse genome, wherein the target sequence comprises a first expression cassette and a second expression cassette located downstream of the first expression cassette,

• wherein the first expression cassette has following elements connected in series in sequence:
• a liver-specific promoter, a tetracycline transcription activation regulating factor and a first polyA;
• and the second expression cassette has following elements connected in series in sequence:
• a second polyA, a mouse prourokinase activator encoding gene and a tetracycline-inducible promoter,
• wherein the liver-specific promoter drives expression of the tetracycline transcription activation regulating factor in a direction from upstream to downstream, and the tetracycline-inducible promoter drives expression of the mouse prourokinase activator encoding gene in a direction from downstream to upstream, wherein the target site is Rosa26 site.