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US 2022/0137061 A1Published application
Systems And Methods For Preparing A Sample and Performing A Real-Time Assay Of The Sample
AssigneeAmgen Inc.
InventorsChao-Hsiang Wu, Gang Xue, Brandon Zachary Sarich, Michael R. Berke, Jacob Sebastain, Pik Chan
Life of this patent
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Abstract
Systems and methods that facilitate the automatic (or substantially automatic) preparation of a sample of a product containing molecules for analysis and automatic (or substantially automatic) performance of an assay of that sample. Thus, the preparation and analysis can be performed substantially in-real time, or, in other words, much more quickly than presently allowed by conventional systems and methods.
Claims26
A method comprising:
- (a) moving a sample comprising molecules from a first vial to a sample loop;
- (b) moving a volume of the sample from the sample loop to a first multi-port valve;
- (c) moving the volume of the sample from the first multi-port valve to a second multi-port valve fluidly coupled to and arranged downstream of the first multi-port valve;
- (d) when the second multi-port valve is in a first position, moving the volume of the sample from the second multi-port valve to a capture column;
- (e) capturing the molecules in the sample in the capture column, thereby separating the molecules in the sample from a matrix of the sample;
- (f) moving an elution buffer solution from a buffer source to the capture column, thereby eluting molecules captured by the capture column and moving an elution/molecule mixture comprising the elution buffer solution and the eluted molecules to a second vial arranged downstream of the capture column, the second vial comprising a flow-through vial;
- and
- (g) moving the molecules in the second vial to an analytical device for analysis of the molecules.
5 more elements
-10. (canceled)
-17. (canceled)
-31. (canceled)
A method comprising the steps of:
- (a) moving a sample comprising polypeptides from a first vial to a sample loop;
- (b) moving a volume of the sample from the sample loop to a first multi-port valve;
- (c) moving the volume of the sample from the first multi-port valve to a second multi-port valve fluidly coupled to and arranged downstream of the first multi-port valve;
- (d) moving the volume of the sample from the second multi-port valve to a polypeptide binding column;
- (e) binding the polypeptides in the volume of the sample to the polypeptide binding column, thereby separating the polypeptides in the sample from a matrix of the sample;
- (f) moving glycosidases to the polypeptide-binding column via the second multi-port valve to release glycans from the bound polypeptides;
- (g) moving the released glycans out of the polypeptide-binding column and to a second vial arranged downstream of the polypeptide-binding column, the second vial comprising a flow-through vial;
- (h) mixing the released glycans with a glycan-labeling reagent in the second vial;
- (i) moving the mixture of the released glycans and the glycan-labeling reagent to a reaction coil via the first multi-port valve;
- (j) incubating the mixture of the released glycans and the glycan-labeling reagent in the reaction coil, thereby labeling the glycans;
- (k) moving the mixture from the reaction coil to a third vial, the third vial also comprising a flow-through vial;
- and
- (l) moving the labeled glycans in the third vial to an analytical device for analysis of the labeled glycans.
10 more elements
-111. (canceled)
A closed system comprising:
- a first vial adapted to contain a sample comprising molecules;
- a sample loop adapted to receive the sample from the first vial;
- a first multi-port valve fluidly coupled to the sample loop and arranged to obtain a volume of the sample via a first port of the first multi-port valve;
- a second multi-port valve fluidly coupled to and arranged downstream of the first multi-port valve;
- a capture column arranged to be fluidly coupled to the second multi-port valve when the second multi-port valve is in a first position, the capture column configured to bind the molecules from the sample;
- a second vial arranged downstream of the capture column;
- and
- a buffer source fluidly coupled to the first multi-port valve and arranged to supply elution buffer solution to the second vial via the second multi-port valve and the capture column when the second multi-port valve is in the first position, such that the elution buffer solution is adapted to elute substantially all of the molecules from the capture column,
- wherein the second vial comprises a flow-through vial configured to filter the elution buffer solution from the elution/molecule mixture out of the second vial, thereby leaving only the eluted molecules in the second vial.
6 more elements
-118. (canceled)
-146. (canceled)
-153. (canceled)
A flow-through vial for use in preparing a sample of a product containing molecules for analysis and performing an assay of that sample, the flow-through vial comprising:
- a base;
- a gutter coupled to and extending outwardly from the base;
- an inlet port formed in the base;
- and
- an outlet port formed in the gutter.
2 more elements
(canceled)
Dependencies are inferred from the claim text.