Method for Optimising the Concentration of Elements of Interest for Visual Measurement on a Biological Sample

An imaging method for imaging a body fluid sample for visual measurement relating to leukocytes of the sample, said method comprising the following steps:

• an acquisition of the leukocyte concentration of a solution derived from the sample, resulting in obtaining a measured concentration of the leukocytes in the sample;
• a dilution of a test solution according to a dilution ratio determined as a function of the measured concentration of leukocytes in the sample, so as to bring the leukocyte concentration of the test solution to an optimal leukocyte concentration;
• a transfer of the test solution into the optical chamber and a rotation of optical chamber by a centrifugation unit so as to align the leukocytes of test solution on an optical plane comprised in the optical chamber, the optical plane being perpendicular to a direction of thickness of the optical chamber, wherein the optimal concentration of leukocytes for the test solution corresponds to a target surface density on the optical plane comprised between 20 leukocytes per square millimetre and 1000 leukocytes per square millimetre after aligning the leukocytes with the optical plane,
• imaging, by an imaging device, of the test solution in the optical chamber.