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AssigneeDupont Nutrition Biosciences Aps
InventorsPeter Edvard DEGN, Xiaogang GU, Karsten Matthias KRAGH, Robin Anton SORG, Steffen Yde BAK, Svend HAANING, Xinyue TANG, Helong HAO, Marc Anton Bernhard KOLKMAN
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The present invention related to a method for preparing a protein hydrolysate from a proteinaceous material by contacting the material with a proteolytic enzyme mixture having a proline specific exopeptidase. In particular, the proline specific exopeptidase is an aminopeptidase specific for at the five amino acid N-terminal sequence X-Pro-Gln-Glv-Pro-, where X is any amino acid. The present invention also relates to use of the aminopeptidase with a second exopeptidase and an endopeptidase.
A method for preparing a protein hydrolysate from a proteinaceous material which method comprises contacting the proteinaceous material under aqueous conditions with a proteolytic enzyme combination comprising an exopeptidase specific for peptides having a proline in the penultimate N-terminus.
The method for preparing a protein hydrolysate according to any preceding claim wherein the proteolytic enzyme mixture further comprises a second exopeptidase.
The method for preparing a protein hydrolysate according any of the preceding claims wherein the proteolytic enzyme mixture further comprises an endopeptidase.
The method for preparing a protein hydrolysate according to any of the preceding claims wherein the proteinaceous material comprises a vegetable derived protein, an animal derived protein, a fish derived protein, an insect derived protein or a microbial derived protein.
The method for preparing a protein hydrolysate according to any of the preceding claims wherein the proteolytic enzyme mixture comprises at least an exopeptidase specific for peptides having a proline in the penultimate N-terminus, a second exopeptidase and an endopeptidase.
The method for preparing a protein hydrolysate according to any of the preceding claims wherein the method is for producing a protein hydrolysate having elevated levels of glutamic acid.
A protein hydrolysate produced according to a method according to any of the preceding claims.
Dependencies are inferred from the claim text.