Modified chloramphenicol acetyltransferase and biosynthesis method of making esters using same
AssigneeUniversity of Tennessee Research Foundation
InventorsCong T. TRINH, Hyeongmin SEO, Jong-Won LEE
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Abstract
A modified chloramphenicol acetyltransferase comprising a tyrosine residue 20 having a phenylalanine (Y20F) mutation, a microorganism harboring the modified chloramphenicol acetyltransferase, and a method of producing ester by feeding the microorganism are disclosed. The method includes providing the microorganism harboring a modified chloramphenicol acetyltransferase in an environment suitable for the microorganism to produce an ester and feeding the microorganism (i) a sugar or a cellulose, and (ii) an alcohol and/or a carboxylic acid.
Claims20
A modified chloramphenicol acetyltransferase comprising a tyrosine residue 20 having a phenylalanine (Y20F) mutation.
A microorganism harboring a modified chloramphenicol acetyltransferase comprising a tyrosine residue 20 having a phenylalanine (Y20F) mutation.
A method of producing esters comprising:
- providing a microorganism harboring a modified chloramphenicol acetyltransferase comprising a tyrosine residue 20 having a phenylalanine (Y20F) mutation in an environment suitable for the microorganism to produce an ester;
- and
- feeding the microorganism a sugar or a cellulose;
- and
- feeding the microorganism an alcohol and/or a carboxylic acid.
Dependencies are inferred from the claim text.