Plant dna methyltransferases and uses thereof
AssigneeRamot at Tel-Aviv University Ltd.
InventorsAssaf ZEMACH, Nir OHAD, Rafael YAARI, Aviva KATZ, Katherine DOMB
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Abstract
An isolated polynucleotide encoding a fusion protein which comprises a DNA targeting moiety linked to a catalytic domain of a plant DNA methyltransferase 3 (DNMT3) protein is disclosed. Uses thereof are also disclosed.
Claims28
An isolated polynucleotide encoding a fusion protein which comprises a DNA targeting moiety linked to a catalytic domain of a plant DNA methyltransferase 3 (DNMT3) protein.
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An isolated polynucleotide encoding a catalytic domain of a plant DNA methyltransferase 3 (DNMT3) protein having a codon usage optimized for expression in an organism which is not a gymnosperm or a bryophyte.
8-9. (canceled)
12-24. (canceled)
28-31. (canceled)
An expression vector comprising a polynucleotide encoding a catalytic domain of a species of a plant DNA methyltransferase 3 (DNMT3) protein operatively linked to a transcriptional regulatory sequence which is not of said species.
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40-42. (canceled)
A method of increasing methylation of DNA in a cell, the method comprising expressing a polynucleotide encoding a catalytic domain of a plant DNA methyltransferase 3 (DNMT3) protein in the cell, thereby increasing methylation of DNA in the cell, wherein the cell is not of a gymnosperm plant.
44-45. (canceled)
47-49. (canceled)
Dependencies are inferred from the claim text.