Protein hydrogel, preparation method and use thereof
AssigneeReal Research Sp. Z O.O.
InventorsMarcin Przemyslaw KRZYKAWSKI, Renata KRZYKAWSKA
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Abstract
The invention relates to a new protein hydrogel created on the basis of low-concentrated components: reagents A and B, the method of hydrogel preparation and its use.
Claims16
1-16. (canceled)
A protein hydrogel comprising:
- reagent A, being gelatin, reagent B, being a cross-linking agent, being GTA, and solvent, characterised in that reagent A is present in the final concentration from 0.15% wt. to 1.5% wt., with a ratio of reagent A to reagent B of 0.375-4.5 mg to 0.01-0.15 mg in one portion of the hydrogel, wherein solvent is dH2O or PBS.
The protein hydrogel according to any one of the preceding claims, characterized in that gelatin is gelatin of the Bloom value of at least 225, preferably of the Bloom value of 300.
The method according to any one of the claims from 21, characterized in that if the purification of the hydrogel in step h) takes place, it takes place by means of rinsing with an aqueous solution, preferably an aqueous solution for cell cultures, preferably PBS, or by means of neutralising reagent B, preferably by adding L-lysine.
Dependencies are inferred from the claim text.