Methods of isolating a protein
AssigneeBristol-Myers Squibb Company
InventorsDouglas D. FREY, Yang LIU, Sevda DELDARI, Ryan Keith SWANSON, Chittoor R. NARAHARI, Ronald Charles BATES, Sanchayita GHOSE, Zhengjian LI
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Abstract
The disclosure provides a method of separating a protein product, e.g., from a contaminant in a mixture, comprising contacting the mixture with a polishing chromatography matrix. In some embodiments, the polishing chromatography matrix is used together with a retained gradient. In certain embodiments, the retained gradient comprises a pH gradient. In certain embodiments, the mixture comprises a monoclonal antibody product of a prior protein A chromatography.
Claims94
A method of separating a protein product from a contaminant in a mixture, comprising contacting the mixture with a polishing chromatography matrix.
A method of polishing a protein product in a mixture comprising the protein product and a contaminant comprising contacting the mixture with a polishing chromatography matrix.
A method of separating a protein product from a contaminant in a mixture, comprising:
- (1) contacting the mixture with a polishing chromatography matrix, and
- (2) applying a retained gradient to the polishing chromatography matrix.
A method of polishing a protein product from a contaminant in a mixture, comprising
- (1) contacting the mixture with a polishing chromatography matrix, and
- (2) applying a retained gradient to the polishing chromatography matrix.
The method of any one of claims 53 to 56, wherein the elution buffer comprises about 0.1 mM to about 20 mM, about 0.5 mM to about 15 mM, about 5 mM to about 15 mM, about 6 mM to about 15 mM, about 7 mM to about 15 mM, about 8 mM to about 15 mM, about 9 mM to about 15 mM, about 10 mM to about 15 mM, about 10 mM to about 14 mM, or about 11 mM to about 13 mM MOPS.
The method of any one of claims 79 to 86, wherein the starting buffer has a pH of about 9.8.
Dependencies are inferred from the claim text.