Method of dna synthesis

An in vitro cell-free process for production of a deoxyribonucleic acid (DNA) which comprises a desired DNA sequence, said process comprising:

• (a) contacting an oligonucleotide immobilised on a solid support with a series of template oligonucleotides which overlap in sequence, in the presence of at least one DNA polymerase under conditions promoting template-dependent extension of said immobilised oligonucleotide to produce a first DNA strand which comprises the desired DNA sequence and further comprises a first portion of the protelomerase target sequence proximal to the solid support;
• (b) introducing a DNA sequence comprising a second portion of the protelomerase target sequence of (a) in the distal part of said first DNA strand produced in (a), or on a second DNA strand, such that said first and second portions of the protelomerase target sequence thereby create a complete target sequence for the protelomerase of (a) proximal to said solid support;
• and
• (c) contacting said complete protelomerase target sequence proximal to said solid support with a protelomerase under conditions promoting cleavage and rejoining of said target sequence, to thereby release the produced DNA from immobilisation.