Engineered cells for improved production of cannabinoids
AssigneeGenomatica, Inc.
InventorsMichael A. Noble, Jonathan Moore, Jingyi Li, Anna Lechner
Life of this patent
Estimating the term…
Abstract
The invention provides non-natural microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing synthesis of olivetolic acid, olivetolic acid derivatives and/or cannabinoids.
Claims29
An engineered cell engineered for the production of olivetolic acid, a derivative of olivetolic acid, or a cannabinoid, wherein the engineered cell is engineered to express or overexpress:
- a. at least one nucleic acid sequence encoding a polypeptide having Type III polyketide synthase activity;
- b. at least one nucleic acid sequence encoding a polypeptide sequence having olivetolic acid cyclase activity that forms olivetolic acid or an olivetolic acid derivative;
- and
- at least one nucleic acid sequence encoding an acetyl CoA carboxylase (ACC) or subunit thereof.
5-11. (canceled)
An engineered cell engineered for the production of olivetolic acid, a derivative of olivetolic acid, or a cannabinoid, wherein the engineered cell is engineered to express an exogenous gene encoding a CoA ligase having activity on acetate, hexanoate or other fatty acid.
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An engineered cell engineered for the production of a cannabinoid, wherein the engineered cell is engineered to express or overexpress:
- a. at least one nucleic acid sequence encoding a polypeptide having Type III polyketide synthase activity;
- b. at least one nucleic acid sequence encoding a polypeptide sequence having olivetolic acid cyclase activity;
- and
- c. at least one nucleic acid sequence encoding a nucleic acid sequence encoding an alcohol kinase, an alcohol diphosphokinase, and/or a prenyl phosphate kinase.
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33-37. (canceled)
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Dependencies are inferred from the claim text.