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AssigneeIntegrated Lipid Biofuels LLC
InventorsXiaochao XIONG, Shulin CHEN
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The present disclosure provides a method for genetically engineering Yarrowia lipolytica host cell for producing glycolic acid from organic wastes. A subject genetically engineered Y. lipolytica cell comprises the disrupted native genes encoding malate synthase, heterologous enzyme of glyoxylate reductase targeted in the different cellular compartments including mitochondria, peroxisome and cytosol, and a mutant NADP+-dependent malate dehydrogenase. The pathway with a theoretical yield as high as that 1 g of acetic acid can be converted to 1.27 g of glycolic acid without carbon loss was engineered for glycolic acid production. The methods particularly include process for production of volatile fatty acids (VFAs) mainly comprised of acetic acid from organic waste, and then use of resultant VFAs for biosynthesis of glycolic acid by recombinant Y. lipolytica.
A system for biosynthesis of glycolic acid, comprising a first expression cassette comprising a polynucleotide encoding glyoxylate reductase operably linked to an expression control sequence and a second expression cassette comprising a polynucleotide encoding a NADP+-dependent malate dehydrogenase operably linked to an expression control sequence.
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A recombinant yeast cell comprising a knockout of at least one malate synthase gene selected from malate synthase 1 (ms1) and malate synthase 2 (ms2).
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A method of producing volatile fatty acids (VFAs) from organic waste, the method comprising inoculating a culture medium with an anaerobic sludge and culturing the anaerobic sludge with the organic waste under anaerobic culture conditions sufficient to convert the organic waste into VFAs.
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Dependencies are inferred from the claim text.