Methods and compositions for ppo herbicide tolerance in plants
AssigneeMonsanto Technology LLC
InventorsArtem G. Evdokimov, Clayton T. Larue, Farhad Moshiri, Joel E. Ream, Xuefeng Zhou
Life of this patent
Estimating the term…
Abstract
The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.
Claims31
A recombinant DNA molecule comprising a heterologous promoter operably linked to a nucleic acid sequence encoding a protein that has at least 85% sequence identity to a polypeptide sequence selected from the group consisting of:
- SEQ ID NOs:1-20, wherein the protein has herbicide-insensitive protoporphyrinogen oxidase activity.
A recombinant polypeptide that comprises at least 85% sequence identity to the full length of an amino acid sequence chosen from SEQ ID NOs:1-20, wherein the polypeptide has herbicide-insensitive protoporphyrinogen oxidase activity.
A method of identifying a nucleotide sequence encoding a protein having protoporphyrinogen oxidase activity, the method comprising:
- a) transforming an E. coli strain having a gene knockout for the native E. coli PPO enzyme with a bacterial expression vector comprising a recombinant DNA molecule encoding a candidate herbicide tolerance protein;
- and
- b) growing said transformed E. coli using a heme-free bacterial medium, wherein growth using said bacterial medium identifies a protein having protoporphyrinogen oxidase activity.
A method of identifying a nucleotide sequence encoding a protein having herbicide-insensitive protoporphyrinogen oxidase activity, the method comprising:
- a) transforming an E. coli strain having a gene knockout for the native E. coli PPO enzyme with a bacterial expression vector comprising a recombinant DNA molecule encoding a recombinant protein;
- and
- b) growing said transformed E. coli using a bacterial medium containing at least one PPO herbicide, wherein growth of bacteria identifies a protein having herbicide-insensitive protoporphyrinogen oxidase activity.
Dependencies are inferred from the claim text.