Inducible Ammonia Production from a Symbiotic Diazotroph, Methods of Creation and Uses Thereof
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsTim Schnabel, Elizabeth Sattely
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AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsTim Schnabel, Elizabeth Sattely
Estimating the term…
The present disclosure describes systems and methods capable of fixing atmospheric nitrogen into bio-available nitrogenous compounds, including ammonia. Embodiments of the present disclosure are directed to synthetic DNA constructs encoding genes to allow release of bio-available nitrogenous compounds in nitrogen fixing diazotrophic organisms. Many of these constructs encode these genes in inducible and constitutive means, such that inducible embodiments can be activated at select times. Additional embodiments are directed to genetically engineered diazotrophs utilizing these constructs to produce bio-available nitrogenous compounds. Further embodiments are directed to methods to create these constructs and organisms as well as to use these constructs and organisms.
An inducible genetic circuit to deactivate glutamine synthetase in an organism comprising:
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An engineered diazotroph to release nitrogenous compounds into the environment comprising:
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A method of increasing bio-available nitrogen in agricultural environment comprising:
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Dependencies are inferred from the claim text.