Lysosomal enzymes modified by cell based glycoengineering
AssigneesUniversity of Copenhagen, Glycodisplay Aps
InventorsClaus KRISTENSEN, Weihua TIAN, Henrik CLAUSEN, Zhang YANG, Sergey VAKHRUSHEV
Life of this patent
Estimating the term…
Abstract
The present invention relates to lysosomal enzymes modified by use of cell based methods, a compositions comprising a modified lysosomal enzyme, as well as methods for producing a modified lysosomal enzyme and therapeutic use of such modified lysosomal enzyme. In particular, the present disclosure relates to a modified lysosomal enzyme which has low Man6P and low exposed Mannose and high sialic acid content of alpha2,3 type enabling long circulation time and improved uptake into difficult-to-reach organs like heart, kidney and brain.
Claims25
A modified recombinant lysosomal enzyme with increased circulation time in plasma as compared to an unmodified version of the same, wherein said enzyme comprises less than 10% mannose-6-phosphate (Man6P) and less than 0.3 mole exposed mannose (Man) per mole of enzyme.
(canceled)
(canceled)
A modified recombinant lysosomal enzyme produced by, said enzyme comprising increased circulation time in plasma as compared to an unmodified version of the same enzyme, wherein said increased circulation time is due to carbohydrate moieties of the enzyme, wherein the carbohydrate moieties reduce binding of the enzyme to at least one of mannose receptors, and/or mannose 6-phosphate receptors, and combinations thereof, and wherein the modified recombinant lysosomal enzyme retains enzymatic activity.
(canceled)
11-15. (canceled)
(canceled)
20-132. (canceled)
Dependencies are inferred from the claim text.