Reduced leaching of a ligand
AssigneeImmunicom, Inc.
InventorsSteven JOSEPHS, Amir JAFRI, Mark RAPTIS
Life of this patent
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Abstract
A column for removal of a component from a fluid is disclosed. The column has a compartment with a cross sectional area. The compartment contains beads having a diameter. A ligand selected to bind to the component is coupled to the beads. The cross-sectional area and bead diameter are selected to maintain a flow velocity of the fluid within the compartment below a first threshold, thereby reducing leaching of the ligand into the fluid. Also described herein is an adsorbent comprising a ligand that is attached to a substrate by an amine bond, wherein the ligand is resistant to dissociation from the substrate.
Claims8
A method of preparing an adsorbent, comprising the steps of:
- activating a substrate by exposing the substrate to an activation solution comprising an inorganic salt;
- forming a bond between the activated substrate and a capture ligand by exposing the substrate to a ligand solution comprising the capture ligand, wherein the ligand solution has a pH in the range of 8.5-10;
- and
- strengthening the bond by exposing the bond to a coupling solution comprising sodium cyanoborohydride, wherein the coupling solution has a pH in the range of 8.5-10 so that the bond forms a secondary amine linkage while avoiding thioether and ether linkages.
An adsorbent, comprising:
- a substrate;
- and
- a capture ligand;
- wherein the adsorbent is prepared by the steps of:
- activating the substrate by exposing the substrate to an activation solution comprising an inorganic salt;
- forming a bond between the activated substrate and the capture ligand by exposing the substrate to a ligand solution comprising the capture ligand, wherein the ligand solution has a pH in the range of 8.5-10;
- and
- strengthening the bond by exposing the bond to a coupling solution comprising sodium cyanoborohydride, wherein the coupling solution has a pH in the range of 8.5-10 so that the bond forms a secondary amine linkage while avoiding thioether and ether linkages.
Dependencies are inferred from the claim text.