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US 2022/0119856 A1Published application
Stall side method for the detection of bacteria in dairy cattle
AssigneeTransformative Technologies
InventorsLawrence SILVER, Lawrence LOOMIS, David DONOVAN
Life of this patent
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Abstract
The present invention relates to several methods to detect gram positive mastitis pathogens in a small sample of bovine milk by luminescence using a combination of specific reagents giving a “cow side” “in-stall” indication of the presence or absence of gram positive mastitis pathogens within a short period of time.
Claims16
1-22. (canceled)
A method for determining amounts of bacteria in bovine milk comprising the steps of:
- i) Contacting the bovine milk with a material which selectively attracts bacteria
- ii) Separating the bacteria from the material and placing the bacteria into solution
- iii) Treatment of the bacterial solution with a bacterial releasing agent capable of lysing bacterial cells to give a released ATP second solution
- iv) Treatment of the second solution with a Luciferin/Luciferase reagent to give a third solution and
- v) Quantitation of bacteria in the third solution by luminescence;
2 more elements
37-55. (canceled)
Dependencies are inferred from the claim text.