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US 2022/0119821 A1Published application
Recombinant host cells and methods for the production of aspartic acid and b-alanine
OwnerJeffrey A. Dietrich, Mario Ouellet +5inventors
InventorsJeffrey A. Dietrich, Mario Ouellet, Ee-Been Goh, Chi Le, Kelvin Shing, Drew Ross, Daniel Blackburn
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Abstract
Methods and materials related to producing aspartic acid, β-alanine and salts of each thereof are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, methods and materials for producing aspartic acid by direct fermentation from sugars are disclosed.
Claims26
A recombinant host cell comprising:
- (a) one or more heterologous nucleic acids encoding an aspartate-forming enzyme either selected from the group consisting of aspartate dehydrogenase and aspartate transaminase or selected from a sequence having at least 95% amino acid identity with SEQ ID NO:
- 23, AspDH #15, AspDH #17, AspDH #18, or AspDH #20;
- and
- (b) one or more heterologous nucleic acids encoding an oxaloacetate-forming enzyme selected from the group consisting of pyruvate carboxylase, phosphoenolpyruvate carboxylase, and phosphoenolpyruvate carboxykinase.
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Dependencies are inferred from the claim text.