Pharmaceutical preparation comprising supernatant of blood mononuclear cell culture and method of use
AssigneeAposcience AG
InventorHendrik Jan Ankersmit
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Abstract
The present invention relates to a topical pharmaceutical preparation for treating an inflammatory skin condition, preferably a condition associated with ischemia, comprising a supernatant of a physiological solution obtainable by cultivating peripheral blood mononuclear cells (PBMCs) or a subset thereof in a physiological solution free of PBMC-proliferating and PBMC-activating substances for at least 1 h.
Claims20
A method of topically treating an ischemia associated inflammatory skin condition comprising:
- administering a topical pharmaceutical preparation to an affected area,
- wherein the topical pharmaceutical preparation comprises a culture supernatant obtained by in vitro cultivation of non-proliferating and non-activated peripheral blood mononuclear cells (PBMCs) comprising at least two of T cells, B cells, or NK cells,
- wherein the PBMCs are cultivated for at least 1 hour in a physiological solution,
- wherein before or during the course of cultivation, the PBMCs are subjected to a stress inducing condition.
A method of topically treating an ischemia associated inflammatory skin condition comprising:
- administering a topical pharmaceutical preparation comprising a culture supernatant including components from a secretome produced in vitro by non-proliferating and non-activated peripheral blood mononuclear cells (PBMCs) comprising at least two of T cells, B cells, or NK cells separated from whole blood,
- wherein the culture supernatant including components from the secretome is obtained by in vitro cultivation of the PBMCs for at least 1 hour within a physiological solution,
- wherein before or during the course of cultivation, the PBMCs are subjected to a stress inducing condition.
A method of topically treating an ischemia associated inflammatory skin condition comprising:
- administering a topical pharmaceutical preparation comprising a culture supernatant produced in vitro by non-proliferating peripheral blood mononuclear cells (PBMCs) comprising at least two of T cells, B cells, or NK cells,
- wherein the culture supernatant includes a non cell-surface moiety triggered secretome production triggered by in vitro cultivation of the non-proliferating PBMCs for at least 1 hour in a physiological solution free of phytohemagglutinin (PHA) and lipopolysaccharide (LPS),
- wherein before or during the course of cultivation, the PBMCs are subjected to irradiation with at least 30 Gy.
Dependencies are inferred from the claim text.