Minimizing immunogenicity of decellularized tissues
AssigneeTissue Testing Technologies LLC
InventorKelvin GM BROCKBANK
Life of this patent
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Abstract
A method for preserving and reducing the immunogenicity of a tissue, the method including obtaining a first tissue, the first tissue being a wild type tissue or genetically modified tissue; forming a second tissue by immersing the first tissue in a first solution having a cryoprotectant concentration of at least about 75% by weight for at least one hour to kill and lyse the cells of the first tissue; forming a third tissue by removing residual cell materials of the second tissue, the residual cell materials of the second tissue being removed by subjecting the second tissue to decellularization in a bioreactor; and subjecting the third tissue to ice-free cryopreservation.
Claims20
A method for preserving a tissue and reducing the immune reaction after transplantation or implantation of the preserved tissue, comprising:
- obtaining a first tissue from a donor, the first tissue being a wild type tissue or genetically modified tissue;
- forming a second tissue by immersing the first tissue in a first solution having a cryoprotectant concentration of at least about 75% by weight for at least one hour to kill and lyse the cells of the first tissue;
- forming a third tissue by removing residual cell materials of the second tissue, the residual cell materials of the second tissue being removed by subjecting the second tissue to decellularization in a bioreactor;
- subjecting the third tissue to ice-free cryopreservation, the ice-free cryopreservation including:
- infiltrating the third tissue with a second solution having a cryoprotectant concentration of at least about 75% by weight by placing the third tissue and the second solution in a container at a predetermined temperature for at least one hour,
- removing the second solution and replacing it with a third solution having a cryoprotectant concentration of at least about 75% by weight, sealing the container after the second solution has been replaced with the third solution such that the sealed container contains the third solution and third tissue, and
- storing the sealed container;
- and
- implanting or transplanting the third tissue into a recipient, wherein
- an immune reaction is not precipitated by the transplantation or implantation of the third tissue, or
- any immune reaction that occurs in the recipient is non-life-threating.
A method for reducing tissue immunogenicity, comprising:
- obtaining a first tissue from a donor, the first tissue being a wild type tissue or genetically modified tissue;
- forming a second tissue by immersing the first tissue in a first solution having a cryoprotectant concentration of at least about 75% by weight for at least one hour to kill and lyse the cells of the first tissue;
- forming a third tissue by removing residual cell materials of the second tissue, the residual cell materials of the second tissue being removed by subjecting the second tissue to decellularization in a bioreactor;
- wherein
- after being transplanted for a predetermined time, such as 1 day, one week, or one year, the third tissue stimulates less of an immune response in humans as compared to a corresponding wild type tissue or genetically modified tissue obtained from the same donor, the corresponding wild type tissue or genetically modified tissue obtained from the same donor being either:
- a tissue that was subject to only decelluarization or cryoprotectant exposure,
- a tissue having reduced immunogenicity that was achieved by via hiding or masking antigens,
- a tissue that was not subject to decellularization and/or cryoprotectant exposure, or
- an unmodified tissue.
A method for preserving a tissue, comprising:
- obtaining a first tissue, the first tissue being a wild type tissue or genetically modified tissue;
- forming a second tissue by immersing the first tissue in a first solution having a cryoprotectant concentration of at least about 75% by weight for at least one hour to kill and lyse the cells of the first tissue;
- forming a third tissue by removing residual cell materials of the second tissue, the residual cell materials of the second tissue being removed by subjecting the second tissue to decellularization in a bioreactor;
- and
- subjecting the third tissue to ice-free cryopreservation, the ice-free cryopreservation including:
- infiltrating the third tissue with a second solution having a cryoprotectant concentration of at least about 75% by weight by placing the third tissue and the second solution in a container at a predetermined temperature for at least one hour,
- removing the second solution and replacing it with a third solution having a cryoprotectant concentration of at least about 75% by weight, sealing the container after the second solution has been replaced with the third solution such that the sealed container contains the third solution and third tissue, and
- storing the sealed container.
Dependencies are inferred from the claim text.