Centrifugal Cassette with Molded Insertable Valves

A Normally Open Valve (NOV) for insertion into a fluidic channel, the NOV comprising:

• a valve base including at least one interference seal member;
• and
• a lumen;
• wherein the at least one interference seal member is configured to cooperate with at least one interference seal member of a valve seat.

A centrifuge cassette assembly for separating a fluid, the cassette assembly comprising:

• a first chamber;
• a second chamber;
• a fluidic channel creating a fluid connection between the first chamber and the second chamber;
• at least one molded insertion valve configured to control the flow of fluid in the fluidic channel;
• and
• a heating element for actuating the at least one molded valve.

A method of manufacturing a centrifuge cassette assembly, the method comprising:

• injection molding a first plate, the first plate including an upper valve seat;
• injection molding a second plate, the second plate including a fluidic channel and a lower valve seat;
• nesting each of the first plate and the second plate in a holding fixture;
• heating elements of the first plate and of the second plate;
• aligning, with one another, the upper valve seat and the lower valve seat;
• pressing the first plate and the second plate together, joining of the plates;
• and
• inserting a valve into the joined plates, the valve engaging each of the upper valve seat and the lower valve seat.