Back to results
US 2022/0112527 A1Published application
Method for biologically producing acetin compound
AssigneesIndustry-Academic Cooperation Foundation Gyeongsang National University, Kyungpook National University Industry-Academic Cooperation Foundation
InventorsSeon Won KIM, Seong Hee JEONG, Kyung Jin KIM, Zada BAKHT
Life of this patent
Estimating the term…
Abstract
A method for biologically producing acetin such as monoacetin, diacetin, or triacetin according to an embodiment of the present disclosure includes reacting acetyl-CoA with glycerol in the presence of a first O-acetyl transferase to obtain the acetin. With the method, acetin which is sustainable and safe, and has more excellent quality while not causing environmental pollution may be obtained.
Claims19
1:
- A method for production of acetin, the method comprising reacting acetyl-CoA with glycerol in the presence of a first O-acetyl transferase to obtain the acetin comprising at least one of monoacetin and diacetin.
2:
- The method according to claim 1, wherein the first O-acetyl transferase is maltose O-acetyl transferase.
3:
- The method according to claim 1, wherein an amino acid sequence of the first O-acetyl transferase has a sequence motif comprising:
- aspartic acid (ASP) at a position corresponding to position 70 of the amino acid sequence of SEQ ID NO:
- 5;
- asparagine (ASN) at a position corresponding to position 84 of the amino acid sequence of SEQ ID NO:
- 5;
- histidine (HIS) at a position corresponding to position 114 of the amino acid sequence of SEQ ID NO:
- 5;
- and
- glutamic acid (GLU) at a position corresponding to position 126 of the amino acid sequence of SEQ ID NO:
- 5.
7 more elements
4:
- The method according to claim 3, wherein an amino acid sequence of the first O-acetyl transferase has a sequence motif comprising:
- tyrosine (TYR) or phenylalanine (PHE) at a position corresponding to position 15 of the amino acid sequence of SEQ ID NO:
- 5;
- arginine (ARG) or glutamine (GLN) at a position corresponding to position 26 of the amino acid sequence of SEQ ID NO:
- 5;
- arginine (ARG) or lysine (LYS) at a position corresponding to position 30 of the amino acid sequence of SEQ ID NO:
- 5;
- and
- phenylalanine (PHE) or tyrosine (TYR) at a position corresponding to position 82 of the amino acid sequence of SEQ ID NO:
- 5.
7 more elements
5:
- The method according to claim 1, wherein the first O-acetyl transferase is composed of any one sequence of SEQ ID NOS:
- 1 to 6.
6:
- The method according to claim 1, further comprising reacting diacetin as a reaction product with acetyl-CoA in the presence of chloramphenicol-O-acetyl transferase or a second O-acetyl transferase, thus to obtain triacetin.
7:
- The method according to claim 6, wherein an amino acid sequence of the second O-acetyl transferase has a sequence motif comprising:
- cysteine (CYS) or leucine (LEU) at a position corresponding to position 31 of the amino acid sequence of SEQ ID NO:
- 7;
- phenylalanine (PHE) or isoleucine (ILE) at a position corresponding to position 102 of the amino acid sequence of SEQ ID NO:
- 7;
- phenylalanine (PHE) or tyrosine (TYR) at a position corresponding to position 143 of the amino acid sequence of SEQ ID NO:
- 7;
- phenylalanine (PHE) or tyrosine (TYR) at a position corresponding to position 166 of the amino acid sequence of SEQ ID NO:
- 7;
- valine (VAL), isoleucine (ILE) or leucine (LEU) at a position corresponding to position 170 of the amino acid sequence of SEQ ID NO:
- 7;
- and
- histidine (HIS) at a position corresponding to position 193 of the amino acid sequence of SEQ ID NO:
- 7.
11 more elements
8:
- The method according to claim 6, wherein the chloramphenicol-O-acetyl transferase is composed of the sequence of SEQ ID NO:
- 7, and the second O-acetyl transferase is composed of any one sequence of SEQ ID NOS:
- 8 to 10.
9:
- A method for production of acetin, the method comprising culturing a microorganism that expresses a gene encoding a first O-acetyl transferase in a medium containing glycerol to produce the acetin.
10:
- The method according to claim 9, wherein the first O-acetyl transferase is maltose O-acetyl transferase.
11:
- The method according to claim 9, wherein an amino acid sequence of the first O-acetyl transferase has a sequence motif comprising:
- aspartic acid (ASP) at a position corresponding to position 70 of the amino acid sequence of SEQ ID NO:
- 5;
- asparagine (ASN) at a position corresponding to position 84 of the amino acid sequence of SEQ ID NO:
- 5;
- histidine (HIS) at a position corresponding to position 114 of the amino acid sequence of SEQ ID NO:
- 5;
- and
- glutamic acid (GLU) at a position corresponding to position 126 of the amino acid sequence of SEQ ID NO:
- 5.
7 more elements
12:
- The method according to claim 9, wherein an amino acid sequence of the first O-acetyl transferase has a sequence motif comprising:
- tyrosine (TYR) or phenylalanine (PHE) at a position corresponding to position 15 of the amino acid sequence of SEQ ID NO:
- 5;
- arginine (ARG) or glutamine (GLN) at a position corresponding to position 26 of the amino acid sequence of SEQ ID NO:
- 5;
- arginine (ARG) or lysine (LYS) at a position corresponding to position 30 of the amino acid sequence of SEQ ID NO:
- 5;
- and
- phenylalanine (PHE) or tyrosine (TYR) at a position corresponding to position 82 of the amino acid sequence of SEQ ID NO:
- 5.
7 more elements
13:
- The method according to claim 9, wherein the gene encoding the first O-acetyl transferase is composed of any one sequence of SEQ ID NOS:
- 16 to 21.
14:
- The method according to claim 9, wherein the microorganism further expresses a gene encoding chloramphenicol-O-acetyl transferase or a second O-acetyl transferase that transfers an acetyl group to diacetin.
15:
- The method according to claim 14, wherein an amino acid sequence of the second O-acetyl transferase has a sequence motif comprising:
- cysteine (CYS) or leucine (LEU) at a position corresponding to position 31 of the amino acid sequence of SEQ ID NO:
- 7;
- phenylalanine (PHE) or isoleucine (ILE) at a position corresponding to position 102 of the amino acid sequence of SEQ ID NO:
- 7;
- phenylalanine (PHE) or tyrosine (TYR) at a position corresponding to position 143 of the amino acid sequence of SEQ ID NO:
- 7;
- phenylalanine (PHE) or tyrosine (TYR) at a position corresponding to position 166 of the amino acid sequence of SEQ ID NO:
- 7;
- valine (VAL), isoleucine (ILE) or leucine (LEU) at a position corresponding to position 170 of the amino acid sequence of SEQ ID NO:
- 7;
- and
- histidine (HIS) at a position corresponding to position 193 of the amino acid sequence of SEQ ID NO:
- 7.
11 more elements
16:
- The method according to claim 14, wherein the gene encoding chloramphenicol-O-acetyl transferase is composed of the sequence of SEQ ID NO:
- 24, and the gene encoding the second O-acetyl transferase is composed of any one sequence of SEQ ID NOS:
- 25 to 27.
17:
- The method according to claim 9, wherein the microorganism includes a gene encoding acetylesterase, which is attenuated or deleted.
18:
- The method according to claim 9, wherein the microorganism further expresses genes encoding glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase (“DL-glycerol-3-phosphatase”), and the medium includes glucose.
19-28. (canceled)