Triterpene Production
AssigneeThe Regents of the University of California
InventorsJay D. Keasling, Yuzhong Liu
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Abstract
An engineered microbial cell expressing a β-amyrin synthase, a cytochrome P450 reductase, a cytochrome P450 C28 oxidase, a cytochrome P450 C16 oxidase and a cytochrome C23 oxidase is used to make quillaic acid from β-amyrin.
Claims20
A method of making an oxidized triterpene from β-amyrin, comprising incubating an engineered microbial cell expressing a β-amyrin synthase, a cytochrome P450 reductase, a cytochrome P450 C28 oxidase, a cytochrome P450 C16 oxidase and a cytochrome C23 oxidase under conditions wherein the C28 oxidase, the C16 oxidase and the C23 oxidize the C28, C16 and C23 carbons, respectively, of β-amyrin to carboxyl, hydroxyl, and formyl (aldehyde), respectively, to form the oxidized triterpene.
An engineered microbial cell for making an oxidized triterpene, the cell expressing a β-amyrin synthase, a cytochrome P450 reductase, a cytochrome P450 C28 oxidase, a cytochrome P450 C16 oxidase and a cytochrome C23 oxidase, wherein the C28 oxidase, the C16 oxidase and the C23 oxidize the C28, C16 and C23 carbons, respectively, of β-amyrin to carboxyl, hydroxyl, and formyl (aldehyde), respectively, forming the oxidized triterpene.
Dependencies are inferred from the claim text.