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US 2022/0106620 A1Published application
Designing customized protein-specific-buffer system
OwnerShawn Clarkinventor
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Abstract
The present invention is related to the field of protein chemistry. In particular, mixed buffer compositions are formulated that allow an accurate identification of agent-induced changes in protein melting point temperatures. Such buffer compositions provide for methods that determine the specific effects of exogenous agents on protein stability, cryoprotective effects and/or protein quality control (e.g., synthesis and/or extraction purity validations).
Claims35
A method for designing a customized protein-specific buffer system, comprising:
- a) providing:
- i) a solid substrate comprising a plurality of testing wells and a control well, wherein each of said plurality of testing wells comprises a first buffer and a second buffer and a different test compound, said control well comprises a sample buffer, and each of said plurality of testing wells and control well comprises a protein sample;
- ii) a thermocycler device configured with an optical reaction module and an optically readable storage medium device;
- b) inserting said solid substrate into said thermocycler device;
- c) cycling said thermocycler device between a first temperature and a second temperature under conditions such that said optical reaction module collects a data file from each of said plurality of testing well series and said control well;
- and
- d) storing said data files on said optically readable storage medium device;
- e) processing said data files to select at least one test compound as a component for a customized buffer system for said protein.
5 more elements
A method for determining protein quality:
- comprising:
- a) providing;
- i) a solid substrate comprising a plurality of testing wells and a control well, wherein each of said plurality of testing wells are prefilled with a first buffer and a second buffer, and a plurality of testing well series of said plurality of testing wells wherein each well of said plurality of testing well series is prefilled with a different hydrogen ion concentration;
- and
- ii) a thermocycler device configured with an optical reaction module and an optically readable storage medium device;
- b) inserting said solid substrate into said thermocycler device;
- c) cycling said thermocycler device between a first temperature and a second temperature under conditions such that the optical reaction module generates a data file from each of said plurality of testing well series and control well;
- d) storing said data files on said optically readable storage medium device;
- e) processing said data files to produce a plurality of protein melting point temperature profiles;
- displaying said protein melting point temperature profiles as a first three dimensional map;
- and
- g) identifying changes in quality of said protein by differences between said first three dimensional map and a control three dimensional map of said protein.
9 more elements
Dependencies are inferred from the claim text.