Use of modified hiv-1 for generating fully human antibodies
AssigneeUniversity of Massachusetts
InventorJeremy LUBAN
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Abstract
The present invention provides highly efficient methods, and compositions related thereto, for generating high titer human antibodies or antibody fragments thereof in a mammalian subject. The methods comprise administering a virus or virus-like particle to a mammal comprising heterologous immune cells and isolating a population of immunoglobulin-producing cells from the mammal, thereby producing the antibodies or antibody fragments thereof.
Claims38
A method of producing antibodies or antibody fragments thereof comprising the steps of:
- a) administering a virus or virus-like particle comprising the nucleotide sequence set forth in SEQ ID NO:15 to a mammal comprising heterologous immune cells;
- and
- b) isolating a population of mammalian immunoglobulin-producing cells from the mammal, thereby producing the antibodies or antibody fragments thereof.
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An isolated nucleic acid comprising a nucleotide sequence at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% identity to SEQ ID NO:1, wherein the isolated nucleic acid comprises at least one nucleotide sequence selected from the group consisting of SEQ ID NOS:2, 3, and 4, or wherein the isolated nucleic acid comprises a nucleotide sequence selected from the group consisting of SEQ ID NOS:5, 6, 7, 8, 9, 10, and 11.
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A method of treating a disease or disorder in a mammal, the method comprising administering a virus or virus-like particle comprising a nucleotide sequence set forth in SEQ ID NO:
- 15 to the mammal, thereby treating the disease or disorder.
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Dependencies are inferred from the claim text.