Reduced leaching of a ligand
AssigneeImmunicom, Inc.
InventorsSteven JOSEPHS, Amir JAFRI, Mark RAPTIS
Life of this patent
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Abstract
A column for removal of a component from a fluid is disclosed. The column has a compartment with a cross sectional area. The compartment contains beads having a diameter. A ligand selected to bind to the component is coupled to the beads. The cross-sectional area and bead diameter are selected to maintain a flow velocity of the fluid within the compartment below a first threshold, thereby reducing leaching of the ligand into the fluid. Also described herein is an adsorbent comprising a ligand that is attached to a substrate by an amine bond, wherein the ligand is resistant to dissociation from the substrate.
Claims24
1-25. (canceled)
A column comprising an adsorbent obtained by the steps of:
- oxidizing a substrate;
- forming a Schiff base between a ligand comprising a portion of Tumor Necrosis Factor alpha (TNF-alpha) that does not comprise an amino acid substitution and the oxidized substrate;
- and
- converting the Schiff base to a secondary amine bond.
A column for removal of a tumor necrosis factor (TNF) receptor from blood or blood fractions, the column comprising:
- a compartment;
- a substrate disposed within the compartment;
- and
- a ligand coupled to the substrate, the ligand comprising:
- at least three components A that comprise a naturally occurring TNF monomer or a functional fragment thereof that does not comprise an amino acid substitution;
- at least two components B that comprise a peptide linker;
- and
- a linker coupled between one of the components A and the substrate.
Dependencies are inferred from the claim text.