Novel adenylosuccinate synthetase and method for producing purine nucleotides using the same
AssigneeCj Cheiljedang Corporation
InventorsMin Ji BAEK, Ji Hye LEE, So-jung PARK, Jee Yeon BAE
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Abstract
The present disclosure relates to an adenylosuccinate synthetase variant, a microorganism containing the same, and a method for preparing purine nucleotides using the microorganism.
Claims10
A polynucleotide encoding an adenylosuccinate synthetase variant having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:2 and having adenylosuccinate synthetase activity, wherein the amino acid corresponding to position 85 of the amino acid sequence of SEQ ID NO:
- 2 is substituted with a different amino acid, wherein the different amino acid is selected from the group consisting of serine, alanine, valine, leucine, methionine, isoleucine, threonine, asparagine, glutamine, cysteine, tyrosine, lysine, aspartic acid, and glutamic acid, and wherein said adenylosuccinate synthetase variant has increased purine production relative to a wild type adenylosuccinate synthetase.
A microorganism of the genus Corynebacterium capable of producing purine nucleotides, comprising an adenylosuccinate synthetase variant having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:2 and having adenylosuccinate synthetase activity, wherein the amino acid corresponding to position 85 of the amino acid sequence of SEQ ID NO:
- 2 is substituted with a different amino acid, wherein the different amino acid is selected from the group consisting of serine, alanine, valine, leucine, methionine, isoleucine, threonine, asparagine, glutamine, cysteine, tyrosine, lysine, aspartic acid, and glutamic acid, and wherein said adenylosuccinate synthetase variant has increased purine production relative to a wild type adenylosuccinate synthetase.
Dependencies are inferred from the claim text.