Novel pasteurella multocida strains and vaccines having hyac and nanp deletions
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsROBERT E BRIGGS, FRED M TATUM
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Abstract
The present invention provides novel attenuated Pasteurella multocida strains that may be used, in live or killed form, to formulate vaccines that are highly protective against P. multocida infection in bovines, other mammals, and in birds. The present invention also identifies the combination of nanP and hyaC gene mutations as key to the provision of such vaccines. When appropriately formulated, antigenic material of numerous other bovine pathogens may be combined with the live attenuated Pasteurella multocida strains, to make effective combination vaccines.
Claims16
An attenuated live Pasteurella multocida (P. multocida) bacterium comprising a hyaC gene with at least one attenuating mutation, and a nanP gene with at least one attenuating mutation.
A Pasteurella multocida (P. multocida) bacterium of serogroup A wherein the wild type hyaC, nanP, and, optionally, one or more of Fis, or Leukotoxin A virulence genes are modified such that the bacterium is attenuated.
An isolated and killed Pasteurella multocida (P. multocida) bacterium or bacterin defective for biosynthesis of hyaluronic acid resulting from a mutation in the hyaC gene, and defective for surface sialylation resulting from a mutation in the nanP gene.
Dependencies are inferred from the claim text.