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US 2022/0098250 A1Published application
Orthogonal protein heterodimers
AssigneeUniversity of Washington
InventorsZibo CHEN, Scott BOYKEN, Sherry BERMEO, Robert A. LANGAN, David BAKER
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Abstract
Disclosed herein are designed heterodimer proteins, monomeric polypeptides capable of forming heterodimer proteins, protein scaffolds including such polypeptides, and methods for using the heterodimer proteins and subunit polypeptides for designing logic gates.
Claims15
1-116. (canceled)
A cell comprising a designed protein logic gate, wherein:
- (a) the designed protein logic gate comprises:
- (i) one or more designed dimerizers, wherein:
- (A) each of the dimerizers comprises a fusion of two monomer subunits from different designed heterodimers connected by a polypeptide linker, wherein each of the monomer subunits comprises a binding interface;
- and
- (B) each of the dimerizers comprises a plurality of hydrophobic residues forming substantially hydrophobic interaction surfaces that regulate the association of the binding interfaces with each other;
- (ii) a first designed target monomer comprising:
- (A) a first effector polypeptide domain;
- and
- (B) one or more subunits, each subunit comprising a binding interface;
- and
- (iii) a second designed target monomer comprising:
- (A) a second effector polypeptide domain, and
- (B) one or more subunits, each subunit comprising a binding interface;
- (b) each binding interface of the one or more dimerizers is capable of a binding interaction via its binding interface to the binding interface of (i) another dimerizer, (ii) the first target monomer, or (iii) the second target monomer, wherein the interaction is by an asymmetric hydrogen bond network, to collectively and cooperatively effect gate activation and thereby effect control over a biological function of the cell.
11 more elements
Dependencies are inferred from the claim text.