Low-Power Mass Interrogation System and Assay For Determining Vitamin D Levels

A process for determining Vitamin D metabolite level in a sample, comprising:

• introducing at least one antibody to the Vitamin D metabolite to the sample, wherein the at least one antibody operates to pull down the Vitamin D metabolite from the sample;
• separating the Vitamin D metabolite from the antibody and delivering the Vitamin D metabolite to a buffer solution;
• introducing the buffer solution with Vitamin D metabolite to a mass spectrometer, wherein the mass spectrometer includes an ionization component for producing individual sample ions from the buffer solution with Vitamin D metabolite and magnet assembly for creating a permanent magnetic field region beginning at a focal point of an ion focusing component for deflecting focused sample ions using zero power to a detector, wherein individual sample ions are deflected to different points along the detector in accordance with an individual mass thereof, and further wherein ions having a predetermined mass are indicative of the Vitamin D metabolite.