Systems, methods, and devices for three-dimensional imaging, measurement, and display of wounds and tissue specimens

An imaging device comprising:

• an excitation light source configured to emit a first radiation capable of exciting a fluorophore;
• a filter configured to prevent passage of reflected excitation light and permit passage of fluorescence emitted by the fluorophore;
• an imaging lens;
• a visible light source configured to emit a second radiation;
• an infrared light source configured to emit a third radiation;
• at least one image sensor configured to detect radiation;
• and
• a processor configured to receive the detected radiation and to output data associated with the detected radiation.

A system for three-dimensional fluorescence-based imaging of a target, comprising:

• at least one excitation light source configured to uniformly illuminate a target surface with a uniform field of excitation light during fluorescent imaging;
• at least one white-light source configured to illuminate the target surface during white light imaging;
• at least one infrared radiation source configured to emit infrared radiation toward the target surface;
• an image sensor;
• a filter configured to permit optical signals responsive to illumination of the target surface with the excitation light and having a wavelength corresponding to one or more of bacterial autofluorescence and tissue autofluorescence to pass through the filter to the image sensor;
• and
• a processor configured to:
• receive optical signals responsive to illumination of the target with the infrared light and, based on the received signals responsive to illumination of the target with the infrared light, generate a three-dimensional map of the target surface,
• receive detected optical signals responsive to illumination of the target surface with excitation light and generate a two-dimensional fluorescence image of the target surface,
• receive optical signals responsive to white light illumination of the target surface and generate a two-dimensional white light image of the target surface, and combine the three-dimensional map, the fluorescence image, and the white light image to generate a three-dimensional image of the target surface.