Microscopy method and microscope for imaging an object

A microscope for imaging an object, the microscope comprising

• an imaging beam path to image the object, which imaging beam path comprises a magnifying objective introducing a first spherical aberration influenced by optical properties of the object and which imaging beam path further comprises a correction optical unit introducing a second spherical aberration wherein the correction optical unit is adjustable with regard to second spherical aberration introduced,
• a drive for adjusting the correction optical unit with regard to second spherical aberration introduced,
• a control device for controlling the drive, and
• a storage device accessible by the control device, which storage device a relationship assigning set values of the correction optical unit to types of object wherein at each set value the correction optical unit introduces the second spherical aberration such that this second spherical aberration at least partly compensates the first spherical aberration occurring with the type of object assigned to that set value and, thus, reduces an overall spherical aberration occurring when imaging the assigned type of object,
• wherein the control device is adapted to determine an actual type of the object, to retrieve from the relationship the set value assigned to the actual type of object, and to control the drive on the basis of the set value to reduce the overall spherical aberration, wherein the relationship hold in the storage device provides assignment of the set value in two stages, namely a first stage being a database holding refractive indices as a function of type of object and a second stage being an interrelation between refractive index and the set value.