Method of producing test strips for colorimetric determination of calcium level in biological fluid using ca-ocpc complex
AssigneeChundo Pharm Co., Ltd
InventorsSung Jin Kim, Abeje Abebayehu Silte
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Abstract
Disclosed is a method of producing a test strip for detecting a calcium level in a body fluid test sample obtained from a biological subject such as a human or animal. Also disclosed is a test strip that is useful for determining the concentration of calcium in a human or animal body fluid test sample based on a colorimetric change, and thus provides early detection of osteoporosis by monitoring the calcium excretion rate in an easy and inexpensive manner.
Claims11
A method of producing a test strip for detecting a calcium concentration in a sample obtained from a biological subject, the method comprising:
- impregnating paper with a primary reagent, followed by drying (a);
- and
- impregnating the paper dried in step (a) with a secondary reagent, followed by drying (b),
- wherein the primary reagent is prepared by adding o-cresolphthalein complexone (OCPC), 8-hydroxyquinoline, sodium lauryl sulphate (SDS), and polyvinylpyrrolidone (PVP) to distilled water, followed by mixing and further adding an organic acid thereto, and
- the secondary reagent is prepared by adding N-cyclohexyl-3-aminopropanesulfonic acid and N-methyl-D-glucamine to distilled water, followed by mixing and further adding Triton X-100 and triethylamine borate thereto.
A method of producing a test strip for detecting a calcium concentration in a sample obtained from a biological subject, the method comprising:
- impregnating paper with a mixed solution of a primary reagent and a secondary reagent, followed by drying,
- wherein the first reagent is prepared by dissolving o-cresolphthalein complexone (OCPC), sodium lauryl sulphate (SDS), polyvinylpyrrolidone (PVP), and a compound for selectively masking magnesium in an organic solvent, followed by further adding an organic acid thereto, and
- the second reagent is prepared by adding a pH buffer for adjusting a pH to 10.0 to 11.0 during the reaction between calcium in the sample and OCPC of the primary reagent, 2,7-bis(2-arsonophenylazo) chromotropic acid, and Triton x-100 to distilled water.
Dependencies are inferred from the claim text.