Reagents and methods for detecting protein lysine lactylation
AssigneeJingjie PTM BioLab (Hangzhou) Co., Ltd.
InventorsYingming ZHAO, Di Zhang, Lunzhi DAI
Life of this patent
Estimating the term…
Abstract
The invention provides an isolated peptide comprising a lactylated lysine and a specific affinity reagent that specifically binds to a lactylated lysine in a peptide. Also provided are a method for detecting a lactylated lysine in a protein or a fragment thereof using the affinity reagent and a method for isolating the affinity reagent.
Claims22
An isolated affinity reagent that binds specifically to a lactylated lysine in a peptide.
6-9. (canceled)
A method for isolating an affinity reagent that binds specifically to a lactylated lysine in a peptide, comprising:
- (a) exposing a protein library to a peptide comprising a lactylated lysine, whereby a protein from the protein library binds specifically to the lactylated lysine and forms a binding complex with the peptide;
- and
- (b) isolating the protein from the binding complex, whereby the isolated protein is the affinity reagent.
A method for isolating an affinity reagent that binds specifically to a lactylated lysine in a peptide, comprising:
- (a) immunizing a host with a peptide comprising a lactylated lysine, whereby the host produces an antibody;
- and
- (b) isolating the antibody from the host, whereby the isolated antibody is the affinity reagent.
24-30. (canceled)
Dependencies are inferred from the claim text.