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US 2022/0091128 A1Published application
System and method for ligand thermal analysis
AssigneePortland State University
InventorsAlbert S. Benight, Matthew W. Eskew
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Abstract
Devices for ligand capture and methods of using the device are disclosed. The ligand may be captured from a sample, such as a plasma sample. Methods of identifying, quantifying, and/or characterizing captured ligands also are disclosed. Computer systems and methods for analyzing thermograms and determining the characteristics of ligands present in a sample are disclosed.
Claims15
A device for plasma ligand capture, comprising:
- a body comprising a substrate material, wherein the body is (i) an elongated body with a polygonal cross-section, or (ii) an annular body;
- a poly(methyl methacrylate) (PMMA) coating on at least a portion of a surface of the body;
- and
- a plurality of retrieval moiety molecules covalently bound to the PMMA coating.
1 more element
A method for drug discovery, comprising:
- (a) combining a quantity of a drug candidate with a quantity of a solution comprising one or more plasma proteins to provide an analysis sample;
- (b) obtaining a thermogram of the analysis sample by differential scanning calorimetry;
- (c) inputting the analysis sample thermogram into a computer system;
- (d) comparing, using the computer system, the analysis sample thermogram to a thermogram of a control sample comprising the solution comprising one or more plasma proteins to provide a comparison, the control sample being devoid of the drug candidate;
- (e) determining, based at least in part on the comparison, whether the analysis sample thermogram exhibits a perturbation;
- and
- (f) if a perturbation is exhibited,
- (i) repeating steps (a)-(e) with one or more additional quantities of the drug candidate;
- and
- (ii) determining, based at least in part on the perturbation, a characteristic of an interaction of the drug candidate with the one or more plasma proteins, wherein the characteristic is a binding constant, reaction enthalpy, binding stoichiometry, binding free energy, binding entropy, or any combination thereof.
7 more elements
Dependencies are inferred from the claim text.